How is Peptide Purity Achieved and Verified?
At peptidesciences.com, we provide peptides that exceed 99% purity. Using state-of-the-art solution and solid phase peptide synthetic technology, Peptide Sciences is able to offer the finest quality peptides and proteins fit for any research study or application. Peptide purity is achieved and verified through uncompromising manufacturing and production processes, quality control measures, and the implementation of both high-performance liquid chromatography and mass spectrometry analysis.
HPLC and Mass Spectrometry
High performance liquid chromatography, or HPLC, is a scientific technique used to separate, identify, and quantify each component in a mixture. It is a superior process that allows highly accurate peptide testing to be accomplished. Mass spectrometry (MS) is a technique used to measure masses within a sample by ionizing chemical species and sorting the ions based on their mass to charge ratio. The results are plotted with the ion signal as a function of the mass to charge ratio. Both methods are highly accurate peptide testing techniques and scientifically prove the purity and identity of peptides ordered from peptidesciences.com.
Peptide Sciences takes great pride in the quality of all of the products we manufacture, and we implement testing at all stages of peptide production at our peptide snythesis lab, verifying our peptides’ sequential fingerprints for precision accuracy in every preparation.
What is the Recommended Peptide Purity Level?
Peptide Sciences provides only the highest purity peptides (>99% pure) for sale for research and development use. However, preparations of peptides synthesized for research by many other manufacturers can vary widely in purity. Occasionally, researchers can wonder what the minimum acceptable level of peptide purity is for their given purpose. Generally, the higher the peptide purity level, the more favorable the preparation; critically, for certain applications (such as in vitro studies or clinical trials), only exceedingly pure peptides will be appropriate (greater than 98% purity). However, there are some applications for which a lower peptide purity would be acceptable. The minimum recommended peptide purity level for a given application will therefore depend upon that specific application. Examples of minimum acceptable purity levels are as follows.
Highly Pure (>95% Peptide Purity)
• In vitro and in vivo studies
• Clinical trials
• Drug studies in which peptides are used as pharmaceuticals
• Cosmetic peptides for cosmeceuticals
• Monoclonal antibody production
• Quantitative protease studies and enzyme studies
• Quantitative receptor – ligand interaction studies
• Quantitative blocking and competitive inhibition studies
• Quantitative ELISA and RIA protocol standard
• Chromatography standard
Mid-range Peptide Purity (>85%)
• Peptide blocking studies (Western blot)
• Phosphorylation studies
• NMR studies
• Cell attachment studies
• Phosphorylation studies
• Semi-quantitative studies of enzyme-substrate interactions
• Epitope mapping tests
• Biological activity testing
Lower Peptide Purity (>70%)
• Peptide arrays
• Performing an ELISA standard for measuring titers of antibodies
• Antigens for polyclonal antibody production or affinity purification
Those peptides with greater purity are appropriate for use in an application with a lower minimum acceptable level of purity. Peptide Sciences provides exceedingly high purity peptides that will meet or exceed all previously stated purity requirements.